Urine-Based HPV 6/16 PCR Detection Kit

Product/Service of Accuvis Bio

More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.

Norgen’s Urine-Based HPV 6/16 PCR Detection Kit is a ready-to-use system for the isolation, detection and differentiation of high-risk HPV 16 and low-risk HPV 6. First, the kit contains components for the rapid isolation of total DNA, including viral DNA, from the urine samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains HPV 6/16 Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right. 

The HPV 6/16 Master Mix contains reagents and enzymes for the specific amplification of either a 391 bp region of high-risk HPV 16 and/or a 280 bp region of low-risk HPV 6. In addition, Norgen’s Urine-Based HPV 6/16 PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. The HPV 6/16 PCR Primer Set and Controls are also available separately for end-point PCR detection.


  • Non Invasive, Non Infectious Sample Source – Urine is not infectious for most human pathogens.
  • Isolate High Quality, Inhibitor-Free DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated viral DNA
  • Rapid Procedure - Isolate, detect and differentiate between HPV 6 and HPV 16 in under 3 hours.
  • Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
  • High Sensitivity and Specificity - The limit of detection is less than 450 VP/mL urine for the high-risk HPV 16 and less than 250 VP/mL urine for the low-risk HPV. 
  • Purchase Primer Set and Controls Separately - The HPV 6/16 Primer set as well as the positive control and negative control can be purchased separately

Contact Card

  • Dr. Abdulla Al Najjar
  • +97125015399
  • +97125828719
  • 15B, Male Section, 2nd Floor, Abu Dhabi University Campus, Al Ain Rd, Abu Dhabi, United Arab Emirates
  • P.O. Box 28961

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